Chronic Diseases Prevention Review (Online ISSN: 2158-0820)

by Gongzeng Luo, Hao Xu

Chronic Diseases Prevention Review 2021 5(17) 1-6; published online  20 March 2021

Abstract:  to investigate the effect of one-stage anterior debridement, autogenous iliac bone graft and titanium mesh support for anterior cervical plate fixation in the treatment of primary suppurative infection of cervical intervertebral space. January 2016 to January 2020, 12 patients with primary suppurative infection of cervical intervertebral space were accepted one-stage anterior debridement, autogenous iliac bone graft and titanium mesh supporting anterior cervical plate fixation in our department, including 9 males and 3 females, with an average age of (age, 42-75, 55.5±10.23). All patients were excluded from specific infection and iatrogenic infection. Hematological examination was performed before operation, include white blood cells, hemoglobin, procalcitonin, erythrocyte sedimentation rate and CRP. Pus was taken for bacterial culture and drug sensitivity during operation, nucleus pulposus tissue. Bone tissue were sent for pathological examination to record operation time and intraoperative blood loss. Sensitive antibiotics were selected according to the results of bacterial culture and drug sensitivity test after operation. Drug sensitivity was recorded WBC, hemoglobin, procalcitonin, erythrocyte sedimentation rate and. JOA score before operation, 1 week after operation, 12 months after operation and bone graft fusion at the last follow-up were recorded. All patients completed the operation successfully and completed the 12-month follow-up. No infection recurrence occurred during the follow-up period, and the bone graft healed well. The mean operation time was (66.17±12.26) min , and the mean blood loss was (53.33±24.25) ml. The average duration of antibiotic use was (35.33±4.29) day, the average JOA score was (7.08±3.2) points before operation, while the average JOA score was (10.17 ± 2.29) points 1 week after operation, and the average JOA score was (12.5 ± 2.35) points 3 months after operation. The JOA score after operation was higher than that before operation (P < 0.05). The final follow-up results showed that the bone graft healed and there was no recurrence of infection. One stage anterior debridement, autogenous iliac bone graft and titanium mesh support, and anterior cervical plate fixation are safe and effective in the treatment of primary suppurative infection of cervical intervertebral space.

by  Bo Liu, Yuna Zhang, Rongye Wang, Fengfeng Nie, Bo Chen

Chronic Diseases Prevention Review 2021 5(17)7-10; published online  23 March 2021

Abstract: To investigate the effect of Forsythia Forsythiae on early inflammatory response in acute spinal cord injury in rats, the mechanism of neuroprotective effect was studied. 60 female adult healthy rats, weight 200-240g. Randomly divided into experimental control group (A group), spinal cord injury group (B group), forsythia fat group (C group). Each group was 20 rats. After anesthesia, A group performed laminectomy to remove the exposed dura mater. The acute rat spinal cord injury model was made by Allen's heavy blow method in B group and C group after exposure to dura mater. After one hour of successful modeling, saline was injected into B group, 10mg/kg, C group was given intraperitoneal injection of forsythia, 10mg/kg. After 24 hours, 10 rats were killed and spinal cord tissue was removed. ELISA method was used to detect TNF-α and IL-6 concentrations in spinal cord tissue. Westernblot detection of protein expression in spinal cord tissue p38MAPK injury, HE staining to observe the tissue morphology after spinal cord injury in acute rats. BBB scores were used to evaluate the motor recovery of both lower limbs in each group at the 1st, 3rd, 7th, 14th, 21th and 28th days after operation. The score of 1st, 3rd, 7th, 14th, 21th and 28th days after operation BBB, A group was significantly higher than that of B, C group (P<0.05). The score of BBB group was higher than that of B group (P<0.05). ELISA tests, The TNF-αand IL-6 concentrations in A group were significantly lower than those in B, C group. C group was significantly lower than B group (P<0.05). Western blot tests, the expression level of p38MAPK protein in A group was significantly lower than that in B
, C group, and the expression level of p38MAPK protein in C group was significantly lower than that in B group (P<0.05). HE results show, A normal nerve cells in the spinal cord, no bleeding, no edema, nerve cell necrosis and mitosis were not observed. Hemorrhage, infiltration of neutrophils and edema of nerve cells were found in B group. C neurons in the spinal cord, mild microglial proliferation, and the lesion was better than B group. Forsythia forsythia lipin inhibits the expression of p38MAPK signaling pathway, reducing inflammatory response and edema after acute spinal cord injury in rats, It has certain protective nerve function.