Chronic Diseases Prevention Review (Online ISSN: 2158-0820)

by  Yongjiang Wang, Yeping Zhang, Jianhua Li, Kaiqiang Qin, Peng Gao

Chronic Diseases Prevention Review 2017 1(2) 1-7; published online 25 April 2017

Abstract: To analyze and compare the expressions of two factors, forkhead box C1 (FOXC1) and E-cadherin, between gastric cancer and paracancerous tissues, and to determine the correlations of the two factor expressions with pathological features, thus providing implications for cancer treatment and prognosis prediction. A total of 102 gastric cancer patients who underwent surgery were randomly included into the current study. After excluding 1 case of other organ cancers and 2 cases receiving radiochemotherapy before surgery, 99 patients became the final subjects. The cancer and paracancerous tissues were dissected and processed into paraffin-embedded sections for two-step biotin-free immunohistochemistry (IHC) detection. The expressions of the two factors were detected, and statistical differences analyzed. The relationships between the expression levels and pathological features were further explored. The positive expression rate of FOXC1 in cancer and paracancerous tissues was 56.16% and 11.54%, respectively; and that of E-cadherin in the two tissue types was 24.14% and 94.20%, respectively. These data indicated that the expression levels of FOXC1 and E-cadherin were significantly different in tumor tissue (P<0.05). The expression of FOXC1 was not significantly different between cases of different ages or genders (P>0.05), but was significantly different between cases with distinct TNM classifications, histological subtypes and invasive depths, and presence or absence of lymph node metastasis (P<0.05). Similarly, the expression of E-cadherin was not significantly different between cases divided by ages or genders (P>0.05), but significantly different between cases divided by TNM classifications, histological subtypes, invasive depths, and lymph node metastasis (P<0.05). The two factors, FOXC1 and E-cadherin, may play important roles in the carcinogenesis, development, invasion and metastasis of gastric cancer, and provide implications for the mechanisms underlying gastric oncogenesis. They may also serve as new indicators of prognosis and potential targets for treatment of gastric cancer. Moreover, FOXC1 expression may also be associated with the downregulation of E-cadherin expression. FOXC1 probably regulates E-cadherin expression, thus affecting epithelial-mesenchymal transition (EMT) in gastric cancer.

by Caihong Xue, Lihui Sun, Xinzhi Shan, Li Guo, Hong Sun, Yanjun Zhao

Chronic Diseases Prevention Review 2017 1(2) 8-13; published online 25 April 2017

Abstract: The purpose of this study was to develop the quality indicators of health education for patients with severe acute pancreatitis (SAP), so as to provide nursing health education quality testing tools. Based on the literature review and a preliminary survey, we identified 3 first-level quality indicators and 26second-level quality indicators of health education for patients with severe acute pancreatitis (SAP). Using a modified Delphi technique, 26 nursing experts that were knowledgeable and experienced in the field of nursing were consulted with two rounds. A questionnaire was developed following 4 steps, and involved ranking indicators on a five-point Likert scale. Mean (M), standard deviation (SD) and coefficient of variation (CV) were used to describe the importance of indicators. The expert agreements were measured using Kendall concordance coefficient (Kendall’s W). The results showed that a consensus was achieved after the preliminary survey and two rounds of modified Delphi technique, with the ultimate containing 3first-level indicators and 23 second-level indicators. All the experts surveyed are authoritative and responded to the investigation; significant experts disagreement was found (Kendall’s W, p < 0.01). By using a modified Delphi technique, we developed the health quality indicators, which provide a basis for clinical nurses management and the monitoring of health education.

by  Yue Yang, Mai Xu, Zhenhua Ma, Xueren Teng

Chronic Diseases Prevention Review 2017 1(2) 14-17; published online 25 April 2017

Abstract: To observe the effect of locking plate and ordinary anatomical type plate internal fixation for the treatment of tibial plateau fracture. 56 cases of tibial plateau fracture were treated with proximal tibial plate from April 2009 to January 2015. 21 cases were treated with locking compression plate and 35 cases were treated with anatomical plate. Operative blood loss,length of hospital stay,fracture time healing ,range of motion, tibial varus angulation, posterior slope angle，HSS score and Rasmussen score were compared after internal fixation for the treatment of tibial plateau fracture between locking compression plate and anatomical plate. All patients were followed up with an average follow-up of 13 months. Locking plate is of no statistically difference in operative blood loss,length of hospital stay,fracture time healing,range of motion, tibial varus angulation, posterior slope angle，HSS score and Rasmussen score. Locking plate is not better than non-locking anatomical plate in treating tibial plateau fractures.

by  Hui Ma, Huimin Lv, Xuerong Li, Hongying Zheng, Qun Wang

Chronic Diseases Prevention Review 2017 1(2) 18-26; published online 25 April 2017

Abstract: To detect the activity and quantity of NK cells in different stages of chemotherapy in children with acute leukem and to explore its significance of immunotherapy. NK cell was detected by flow cytometry with CD107a labeling antibody in peripheral blood from 16 cases of children with newly diagnosed acute leukemia and 46 cases of children with complete remission (CR). 15 cases of healthy children were used as normal control group. 46 cases of children with CR were divided into three groups according to duration of treatment, including 16 cases with CR last for 6-9 months (CR-A group), 14 cases last for 1-1.5 years (CR-B group), 16 cases with CR over 2 years (CR-C group). The quantity and activity of NK cells in newly diagnosed group were significantly lower than the control group (P<0.05). The quantity in CR group was (7.01±2.76)%, and was significantly higher than newly diagnosed patients (P<0.05). The quantity of NK cells in CR-A group, CR-B group, CR-C group was significantly higher than the newly diagnosed group (P<0.05), but there was no significant difference (P>0.05) between CR-A group and CR-B group. The CD 107a labeling antibody by flow cytometry and LDH release assay were positively correlated and the correlation coefficient was 0.754. CD107a labeling antibody by flow cytometry correlates with the LDH releaseassay positively, and its specific, simple, reproducibility detection method. The quantity and activity of newly diagnosed group were lower than normal controls, with time goes by, they were increased after CR 6-9 months and one year, but they are still lower than the normal even CR 2 years later.

by  Jun Liu, Weina Jiang, Hua Chen

Chronic Diseases Prevention Review 2017 1(2) 27-31; published online 25 April 2017

Abstract: To investigate the expression of PES1 and EGFR in gastric cancer, and then to explore the relationship between their expression and some clinicopathologic parameters. The expression of PES1 and EGFR was detected by immunohistochemistry in 60 cases of gastric cancer. The positive expression of PES1 and EGFR protein in gastric cancer were significantly higher than those in adjacent tissues (P<0.05). The high expression of PES1 is related to tumor invasive depth and clinical stage (P<0.05, respectively), and the high expression of EGFR was closely related with the depth of invasion, clinical stage and lymph node metastasis (P<0.05, respectively). PES1 and EGFR may play an important role in gastric cancer development, which may be potential targets for the treatment and prognostic evaluation of gastric cancer.

by  Meng Li, Wensi Wu, Xiaotong Liu, Linhao Xu

Chronic Diseases Prevention Review 2017 1(2) 32-36; published online 25 April 2017

Abstract: In this work, a quantitative structure–activity relationship (QSAR) model was used to predict the Mer Specific tyrosine kinase inhibitors by the gene expression programming (GEP). A data set of 43 compounds was used. First, we used the HM to select the descriptors and set up linear models. The result shows that R²=0.7140, F=14.98 S2=2.1697 .Then the data set was divided into two subsets of training and testing. Gene Expression Programming (GEP) was applied to select the respective variables to build nonlinear QSAR models. The GEP method produced a nonlinear and five descriptor quantitative models with a mean error and a correlation coefficient of 0.173 and 0.9549 for the training set, 6.499 and 0.774 for the test set, respectively. Thus the QSAR model will have a better evaluation in providing guidance for designing and synthesizing of anti-thrombosis drugs in the future.