Chronic Diseases Prevention Review (Online ISSN: 2158-0820)
Current Issue
Vol.3 No.12
Article: Vivo study of MSCs on EPCs cells in ischemic heart disease
by Jianming Wang, Gengbiao Chen, Youfeng Guo, Xiaohong Yin, Xiaodong Wang
Chronic Diseases Prevention Review 2019 3(12) 1-5; published online 18 July 2019
Abstract:
The treatment of ischemic heart disease mainly depends on
the formation of blood vessels and the survival of cardiac
myocytes. This paper will research the enhancement of EPC
cells by mesenchymal stem cells in the treatment of ischemic
heart disease. Mouse bone marrow was collected, MSCs cells
were isolated and cultured. Flow cytometry was used to test
the expression of CD34 and CD44. Then MSCs cells and
endothelial progenitor cells co-culture 48 h, and 24 Wistar
rats were randomly divided into EPCs group, EPCs MSCs +
group and the control group. MSCs 2×106, EPCs 2×106, EPCs
2×106, and PBS buffer were injected into five different
regions around ischemic myocardium. The changes in cardiac
function were assessed by echocardiography after 4 weeks.
MSCs 2×106, EPCs 2×106, EPCs 2×106, and PBS buffer were
injected into five different regions around ischemic
myocardium. The changes in cardiac function were assessed by
echocardiography after 4 weeks. The rats were sacrificed,
and the expression of VEGF was detected by
immunohistochemistry and immunofluorescence. The surface
markers CD34 and CD44 of MSCs and EPCs were differentially
expressed. We successfully isolated MSCs and EPCs cells.
Meanwhile, the proliferation index Ki67 was highly
expressed, suggesting that MSCs could effectively promote
the proliferation activity of EPCs. Echocardiography showed
that after MSCs+EPCs treatment, the left ventricular
ejection fraction, end-diastolic volume and cardiac volume
of the rats were significantly improved, with statistically
significant differences from the control group and the EPCs
group (P<0.05), Meanwhile, the expression of VEGF in
MSCs+EPCs group was also significantly increased (P<0.05).
Mesenchymal stem cells can enhance the therapeutic effect of
EPC cells in ischemic heart disease, which can promote
myocardial angiogenesis, improve ventricular remodeling and
cardiac function.
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Article: Longitudinal analysis of trends and reporting quality of statistical methods of observational studies published in high-impact urology journals: a protocol for systematic assessment
by ShuangYang Dai, BeiBei li, JinGao Zhang, XiaoBin Zhou
Chronic Diseases Prevention Review 2019 3(12) 6-14; published online 18 July 2019
Abstract:
Observational studies play an important role in urology
research. The quality of statistical reporting can directly
affect the reliability of observational studies. According
to previous studies, the quality of statistical reporting
for published biomedical literature tends to be low. There
are no studies on the evaluation of the quality of
statistical reports on urological observational studies. The
purpose of this study is to investigate the trends and
evaluate the reporting quality of statistical methods of
observational study published in high-impact urology
journals, compare with general medical journals in order to
further identify factors related to high quality statistical
reporting. This study plans to search the top five journals
of urology and top two general medical journals with the
highest 5-year impact factor (based on ISI Web of Knowledge
Journal Citation Reports) in Pubmed and EMBASE. All
observational studies that meet the inclusion criteria will
be included. Table “common statistical methods in medical
studies” will be used to assess the frequency and trends of
statistical methods for observational studies published in
urology journals. The quality of statistical reporting for
each article will be assessed according to Table “the
assessment checklist of the reporting quality of statistical
methods”. The table consists of 7 items (marked *) that must
be reported and 39 items that are subject to selective
reporting based on the statistical methods used in the
article. The proportion of fully reported items will be
calculated based on the type of statistical method used in
each article. A logistic regression model will be
established to identify factors associated with high-quality
statistical reporting. This study will identify major
deficiencies of statistical reports, which may provide a
reference for authors, reviewers and editors of journals,
and help to improve the statistical reporting quality of
articles in urological journals.
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Article: Analysis on influencing factors for the cure of pulmonary tuberculosis patients in adolescents
by Yuxia Sun, Jianwen Zhang, Chongxiu Sun, Mengru Jia, Yanyan Kang, Aiguo Ma
Chronic Diseases Prevention Review 2019 3(12) 15-19; published online 18 July 2019
Abstract:
To explore the influencing factors of adolescent
tuberculosis patients, and to provide a basis for improving
the cure rate of adolescent tuberculosis patients. The
clinical data of adolescent tuberculosis patients aged 14-35
in the tuberculosis control centers of 7 counties in Linyi
area of Shandong Province from 2009 to 2013 were collected.
Logistic regression model was used to analyze the
influencing factors of adolescent tuberculosis patients. A
total of 706 adolescent pulmonary tuberculosis patients were
included in this study, of whom 669 were cured and 37 were
not cured. Multiple Logistic regression analysis showed that
pulmonary cavity (OR=3.190, 95%CI=1.433-7.054),
complications (OR=8.826, 95%CI=3.327-23.414), sputum test
results at the end of second month (OR=0.112,
95%CI=0.033-0.386) and treatment classification (OR=13.737,
95%CI=4.682-40.304) could affect the cure of pulmonary
tuberculosis in adolescents. We should raise attention for
adolescent tuberculosis patients. During the treatment of
tuberculosis, evaluate and actively intervene in adolescent
tuberculosis patients with pulmonary cavity, comorbidities,
positive sputum test at the end of second month and
retreatment, so as to effectively improve the cure rate of
adolescent tuberculosis patients and prevent the spread and
prevalence of tuberculosis.
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Article: Prevalence and related risk factors of hypertension and normal-high blood pressure in patients with type 2 diabetes mellitus admitted to hospital: a cross sectional study
by Xiuping Du, Shuhui Nie, Xiang Li, Xiangling Cai, Qiuzhen Wang
Chronic Diseases Prevention Review 2019 3(12) 20-27; published online 18 September 2019
Abstract:
With the increase of China's population and economic
development, diabetes (DM) has become a serious public
health problem, the complications of DM are also serious.
Among them, patients with DM complicated with hypertension
have to face the double risk of infection and cardiovascular
disease. Not only the cost of medical care has increased,
but also the quality of life has declined. The purpose of
this study was to compare the differences in lifestyle and
body characteristics between patients with DM complicated
with hypertension, DM with normal-high blood pressure and
patients with DM without normal-high blood pressure, and to
provide clues for the inference of risk factors related to
DM complicated with hypertension. A cross-sectional study
was conducted T2DM patients hospitalized in a hospital from
January 2017 to December 2017. One-way ANOVA and chi-square
test were used to judge the difference between the two
groups. A total of 79 patients with simple DM, 92 patients
with normal-high blood pressure and 138 patients with DM
complicated with hypertension were enrolled in our study. We
found differences in age, neutrophil count, lymphocyte
ratio, uric acid (UA), creatinine (CRE), total protein (TP),
glycosylated hemoglobin (HbA1c), body mass index (BMI),
course of disease and self-control between the two groups.
We found that the age, body mass index (BMI) and
inflammatory response in diabetic patients with hypertension
were significantly higher than those in patients with simple
DM and DM with normal-high blood pressure. However, the
self-control of patients with hypertension is better than
that other groups. Therefore, with the prolongation of
illness time, patients need to control the occurrence of
complications through self-control and weight loss.
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Article: Glucose fluctuation during insulin therapy in diabetic patients with nasal feeding analysis of influencing factors
by Wane Zhao, Xiaohua Lu, Yan Zhou, Jiewei Huang, Chuangbiao Zhang, Yan Liu, Yu Liu, Xixi Luo
Chronic Diseases Prevention Review 2019 3(12) 28-33; published online 28 September 2019
Abstract:
To explore the factors related to blood sugar fluctuation in
diabetic patients with continuous nasal feeding during
insulin pump therapy, and to provide clinical reference for
the adjustment of insulin therapy. Forty diabetic patients
with continuous nasal feeding treated with insulin from
January 2016 to June 2019 in our hospital were selected. The
blood sugar fluctuation was observed by 7-8 fingertip blood
monitoring every day. The blood sugar fluctuation of
patients with continuous nasal feeding was observed by
analyzing the clinical data. In addition, the related
factors affecting blood sugar fluctuation were summarized.
Using LAGE and SDBG as the evaluation indexes of blood sugar
fluctuation, logistic multiple regression analysis of single
and multiple factors was carried out for the possible
related factors affecting blood sugar fluctuation.The blood
sugar levels of different sex, age, course of disease,
glycosylated hemoglobin, body mass index (BMI) and other
indicators of patients were compared, and no statistical
difference was found in the blood sugar fluctuation levels
of gender, age and BMI groups (p>0.05). With the increasing
of diabetes course, the blood sugar fluctuation amplitude
increased (P < 0.05). Carbohydrate content and total protein
during the period also lead to blood sugar fluctuation (P <
0.05). Unconditional multivariate logistic regression
analysis showed that duration (OR = 1.201, P = 0.016),
carbohydrate (OR = 1.029, P = 0.016), total protein (OR =
1.134, P = 0.046) were the factors of blood sugar
fluctuation in diabetic nasal feeding patients. For diabetic
patients with continuous nasal feeding, the fluctuation of
blood sugar is related to the course of disease,
carbohydrate content, total protein and other factors. For
those patients with risk factors, we should take relevant
measures and pay attention to the fluctuation of blood
sugar.
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Article: Sedative effects of right breast feeding chloral hydrate retention enema in MRI examination of infants
by Chunhong Luo, Wencai Yang, Chunliu Luo
Chronic Diseases Prevention Review 2019 3(12) 34-38; published online 28 December 2019
Abstract:
To explore the application effect of chloral hydrate
retention enema in MRI examination of sedation with the
right breast-feeding position of parents and the depth of
anal canal insertion. From June 2017 to June 2018, 98
infants who were sedated by chloral hydrate retention enema
in the medical imaging center of our hospital were randomly
divided into observation group and control group by using
random number table method. In the observation group, the
right breast-feeding position of the parents was adopted,
and the insertion depth of the anal canal was increased to
perform chloral hydrate retention enema. After enema, the
parents did not change the position of the breast-feeding
children. The control group was treated with chloral hydrate
enema by traditional retention enema. The drug spillage,
average sleep time, Ramsay Sedation score and braking effect
were compared between the two groups. The overflow rate of
enema fluid in the observation group was less than that in
the control group(P < 0.05), the onset time of sedation
after enema was significantly faster than that in the
control group(P < 0.05), and the average sleep time was less
than that in the control group(P < 0.05). Ramsay Sedation
score and braking effect were better than those in the
control group, with a statistically significant difference
(P<0.05). The modified chloral hydrate retention enema with
right breast-feeding position of parents and increased
insertion depth of anal canal can reduce the anxiety and
fear of children, effectively reduce the spillover of liquid
medicine, which is easy to operate, practical and better
than the traditional retention enema.
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Article: Early Screening and Diagnosis of Autism
by Zhongjie An
Chronic Diseases Prevention Review 2019 3(12) 39-44; published online 28 December 2019
Abstract:
Autism Spectrum Disorders have for some time been the focus
of intense interest for clinicians and researchers because
of the high prevalence of the disorders among children in
the community. Particular attention has been paid to the
early diagnosis of the disorder and to the intensive
therapeutic intervention. Currently the best prognosis for
autism lays in the early diagnosis and intervention. But
diagnosis of autism at an early age is challenging due to
the existing phenotypic and etiological heterogeneity among
ASD individuals. There are many screening scales for autism,
but their specificity and sensitivity vary to varying
degrees. Therefore, an efficient early diagnosis method is
needed. This paper presents a review of the early detection
of ASD.
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Article: Quantitative structure activity relationship study on the carboxamides derivatives as mosquito repellents
by Fucheng Song, Wei Wang, Xiaofang Ma, Binghui Li, Hongrong Jiang, Hongzong Si, Honglin Zhai
Chronic Diseases Prevention Review 2019 3(12) 45-56; published online 14 December 2019
Abstract:
Molecular field quantitative structure activity relationship
could be used as complementary computational tools for the
analysis of repellents for mosquito. Analysis of these
compounds will help to screen the viable candidates for
further hit-to-lead and lead optimization effort. All
carboxamides derivatives molecular descriptors were
calculated by CODESSA software and total four molecular
descriptors were selected to build function equations.
Heuristic method (HM) and gene expression programming (GEP)
algorithm were used to establish linear and nonlinear
models. The R2 of HM in training and testing sets
are 0.89 and 0.83, the R2 of GEP in training and
testing sets are 0.94 and 0.87, respectively. Wiener index,
Max valency of a H atom, Max antibonding contribution of a
MO and Min resonance energy for a C-N bond can affect the
minimum effective dosages of repellents. Both two models
have potent capability of prediction but the GEP is
obviously superior to HM. This study provides a new and
effective method for mosquito repellents design and
screening.
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