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Cancer Cell Research

Article: B7-H1 Blockade Enhanced the Function of Peripheral Blood Monocyte-Derived Dendritic Cells in Patients with Bladder Cancer

by Xianglong Li, Shixiu Shao, Liran Zhai, Xuewen Guo, Haitao Niu, Yonghua Wang

Cancer Cell Research 2016 3(12) 286-289; published online 22 Octber 2016

Abstract: To investigate B7-H1 expressions on peripheral blood monocyte-derived dendritic cells (DCs) in patients with bladder cancer and the effect of B7-H1 blockade on DCs function. Monocyte-derived DCs (MoDCs) were generated from peripheral blood mononuclear cells, which were obtained from 30 patients with bladder cancer and 10 healthy donors as controls. The expressions of B7-H1 on MoDCs and PD-1 on CD8+T cells were analyzed by flow cytometry. The effects of B7-H1 blockade on MoDCs capacity of stimulating T-cell proliferation and producing IL-10 and IL-12 were detected by mixed lymphocyte reaction and ELISA respectively. Compared with control, the capacity of MoDCs stimulating T-cell proliferation was significantly decreased in patients with bladder cancer. B7-H1 and PD-1 expressions were significantly up-regulated on MoDCs and CD8+T cells respectively in patients with bladder cancer. Moreover, pre-incubation MoDCs with B7-H1 blocking mAb to block B7-H1 pathway resulted in a significant increase in T-cell stimulated proliferation and IL-12 secretion, while a significant decrease in IL-10 secretion. B7-H1 up-regulation may contribute to the functional deficiency of DCs in patients with bladder cancer, while B7-H1 blockade can restore and enhance DCs function in patients with bladder cancer.

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Article: The Influences of Chemotherapeutics on the Cell Cycle in SK-N-SH Neuroblastoma Cells and Clinic Significance

by Xilin Xiong, Ling Liu, Kai Qi, Chi Zhang, Yueqin Chen, Yang Li

Cancer Cell Research 2016 3(12) 294-298; published online 22 Octber 2016

Abstract: This study was aimed to confirm the drug influence on cell cycle of SK-N-SH cells by flow cytometry analysis. The SK-N-SH cells were divided into two groups including Treatment group and control group. All of them were given chemotherapy drugs of ADM, CTX, VP16 and VCR, and then detected the influences of chemotherapy drugs on the cell cycle of SK-N-SH cells by flow cytometry instrument and. ADM: The percentage of G0/G1 phase of experimental group is (51.41±0.13)%, the percentage of G0/G1 phase of control group is (16.59±0.52)%. CTX: The percentage of G0/G1 phase of experimental group is (35.19±0.69)%, the percentage of G0/G1 phase of control group is (9.03±0.21)%. VP16: The percentage of G0/G1 phase of experimental group is (50.25±2.06)%, the percentage of G0/G1 phase of control group is (19.33±1.12)%. DDP: The percentage of G0/G1 phase of experimental group is (25.24±0.37)%, the percentage of G0/G1 phase of control group is (13.51±0.39)%. After treated with ADM, CTX, VP16 and DDP, the cell number of G0/G1 phase is more than control group in SK-N-SH cells, this showed significant difference (P<0.01). VCR: The percentage of G2/M phase of experimental group is (45.76±0.39)%, the percentage of G2/M phase of control group is (10.95±0.36)%. The cell number of G2/M phase is more than control group in SK-N-SH cells, this showed significant difference (P<0.01). ADM, CTX, VP16 and DDP can significantly arrest cell in G0/G1 phase, while VCR can significantly arrest cell in G2/M phase.

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Chronic Diseases Prevention Review

Article: Case-control Study on Serum Calcium and Magnesium Levels in Women Presenting with Preeclampsia

by Xiaoshu Guo, Lin Xu, Junwei Huang, Min Zhao

Chronic Diseases Prevention Review 2017 1(1) 1-6; published online 7 January 2017

Abstract: To study the difference of serum calcium (Ca2+) and magnesium (Mg2+) levels between preeclampsia (PE) pregnant women and normal pregnancy, and the effects on uterine artery blood flow resistance. This case-control study was conducted on 360 pregnant women (≥20 weeks gestation) received antenatal care at Affiliated Hospital of Qingdao University. The cases include 80 pregnant women with mild preeclampsia (MPE), 120 women with severe preeclampsia (SPE). The control group has 160 healthy and age matched pregnant women who were not received mineral during pregnancy. Demographic, anthropometric, clinical and obstetric data were gathered by an interview-based questionnaire. Venous blood samples were drawn for the measurement of serum Ca2+ and Mg2+ level. Doppler ultrasound was used to reflect the dynamic change of uterine artery. Pregnant women with SPE have significantly lower serum Ca2+ and Mg2+ levels than control group and pregnant women with MPE (OR=39.67, P<0.05). The uterine artery blood flow resistance in SPE is higher than others (P<0.05). This study shows that hypocalcemia and hypomagnesemia are the risk factors of SPE. Uterine artery blood flow resistance may be related to the decreased concentration of serum Ca2+ and Mg2+ levels.

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Article: Discussion of Lower Limb Flexor Spasticity in Stroke Patients by Single Point of Continuing Hands Deep Thumb Pressure Rubbing Method

by Dehui Zhu, Ze Wang, Chuanjian Yi, Xiqin Liu, Xiaojun Ji

Chronic Diseases Prevention Review 2017 1(1) 7-9; published online 7 January 2017

Abstract: To observe the curative effect of the single point of continuing hands deep thumb pressure rubbing method to the stroke patient with lower limb flexor spasticity. After seeking for spastic muscle, the patient was treated with the method of a single point of continuing hands deep thumb pressure rubbing method. The treatment was 3~5 seconds with the single point of continuing hands deep thumb pressure rubbing method, and the patient had a rest for 6~10 seconds after the treatment, twice a day, 35 minutes each time. The spastic muscle of the patient had a great improvement. And the patient could self-care after treatment. The single point of continuing hands deep thumb pressure rubbing method has obvious effect on the lower limb flexor spasticity of stroke patients.

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